May 19, 2022 · In vivo continuous evolution is a form of directed evolution that takes advantage of cycles of rapid mutation, amplification and selection inside living cells.

Nov 8, 2024 · We encoded a maladapted tryptophan synthase subunit, TrpB, onto OrthoRep in Saccharomyces cerevisiae and continuously evolved it to gain and maintain the ability to synthesize …

Feb 11, 2025 · In vivo mutagenesis enriches genetic polymorphism within cells, which is pivotal for triggering continuous evolution. Remarkable strides have been made in this field.

In this opinion, we will briefly discuss the current state of continuous evolution systems and present their early successes and potential in reinvigorating the use of directed evolution to study basic questions …

May 6, 2020 · We present automated continuous evolution (ACE), a platform for the hands-free directed evolution of biomolecules. ACE pairs OrthoRep, a genetic system for continuous targeted …

May 23, 2024 · In this review, we summarize the working mechanisms of recently developed continuous evolution strategies, discuss examples of their applications focusing on enzyme evolution, and point …

Continuous Evolution refers to a method used in studying gene adaptation by carrying out controlled forward evolution experiments to discover and map fitness landscapes, test adaptation …

May 22, 2020 · Continuous directed evolution methods allow the key steps of evolution—gene diversification, selection, and replication—to proceed in the laboratory with minimal researcher …

Aug 7, 2025 · We demonstrated continuous evolution using the T7 replisome by expanding the substrate scope of TEM-1 β-lactamase and increasing activity 5000-fold against clinically relevant …

Establishing a continuous cycle of translation, mutation, selection, and replication that is general for a range of biomolecules is challenging, and therefore most continuous evolution methods perform …